Investigating effects of sample pretreatment on protein stability using size-exclusion chromatography and high-resolution continuum source atomic absorption spectrometry

field_of_study

Blank

university

Al-Azhar University – Gaza

authors

Rakow T.; El Deeb S.; Hahne T.; El-Hady D.A.; AlBishri H.M.; Wätzig H.

authors_full_names

Rakow, Tobias (56398061800); El Deeb, Sami (14059838300); Hahne, Thomas (54884842300); El-Hady, Deia Abd (6506274654); AlBishri, Hassan M. (55933386600); Wätzig, Hermann (55963552800)

authors_id

56398061800; 14059838300; 54884842300; 6506274654; 55933386600; 55963552800

year

2014

source_title

Journal of Separation Science

article_title

Investigating effects of sample pretreatment on protein stability using size-exclusion chromatography and high-resolution continuum source atomic absorption spectrometry

volume

37

issue

18

page_start

2583

page_end

2590

publisher

Wiley-VCH Verlag

affiliations

Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethoven Strasse 55, Braunschweig, D-38106, Germany; Department of Pharmaceutical Chemistry, Al-Azhar University-Gaza, Gaza, Palestine; Chemistry Department, Faculty of Science-North Jeddah, King Abdulaziz University, Jeddah, Saudi Arabia; Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

authors_with_affiliations

Rakow T., Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethoven Strasse 55, Braunschweig, D-38106, Germany; El Deeb S., Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethoven Strasse 55, Braunschweig, D-38106, Germany, Department of Pharmaceutical Chemistry, Al-Azhar University-Gaza, Gaza, Palestine; Hahne T., Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethoven Strasse 55, Braunschweig, D-38106, Germany; El-Hady D.A., Chemistry Department, Faculty of Science-North Jeddah, King Abdulaziz University, Jeddah, Saudi Arabia; AlBishri H.M., Chemistry Department, Faculty of Science-North Jeddah, King Abdulaziz University, Jeddah, Saudi Arabia, Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Wätzig H., Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethoven Strasse 55, Braunschweig, D-38106, Germany

cited_by

5

doi

10.1002/jssc.201400189

author_keywords

Adsorption; Atomic absorption spectrometry; Protein stability; Size exclusion chromatography

index_keywords

Adsorption; Analytic Sample Preparation Methods; Animals; Antibodies, Monoclonal; Cattle; Chromatography, Gel; Chromatography, High Pressure Liquid; Immunoglobulin G; Myoglobin; Ovalbumin; Particle Size; Protein Stability; Proteins; Serum Albumin, Bovine; Spectrophotometry, Atomic; Temperature; Absorption spectroscopy; Adsorption; Atomic absorption spectrometry; Atoms; Containers; Freezing; Liquefied gases; Mammals; Monoclonal antibodies; Storage (materials); bovine serum albumin; monoclonal antibody; myoglobin; ovalbumin; solvent; immunoglobulin G; protein; Adsorption of proteins; Bovine serum albumins; Chromatographic column; Chromatographic process; High performance size exclusion chromatography; High-resolution continuum source atomic absorption spectrometry; Investigating effects; Protein stability; adsorption; analytic method; Article; atomic absorption spectrometry; environmental temperature; freezing; gel permeation chromatography; protein stability; storage temperature; analysis; animal; atomic absorption spectrometry; bovine; chemistry; high performance liquid chromatography; particle size; size exclusion chromatography; temperature; Size exclusion chromatography